phosphorylated p abl  (Cell Signaling Technology Inc)

Journal: Genes & Cancer

Article Title: BCR: a new target in resistance mediated by BCR/ABL-315I?

doi:

Figure Lengend Snippet: A. Western blotting on lysates of 32D cells expressing the indicated transgenes using antibodies directed against Erk1/2, phosphorylated Erk1/2. B. Western blotting on lysates of 32D cells expressing the indicated transgenes using antibodies directed against signal transducer and activator of transcription (STAT5) and phosphorylated STAT5. Molecular mass references (KDa) are given and Tubulin was used as a loading control.

Article Snippet: The following antibodies were used: anti-ABL (a-ABL) Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-phosphorylated ABL specific for the phosphorylated tyrosine-residues 245 (a-p-ABL-Y245) (Upstate-Biotechnology, Lake Placid, NY, USA), anti-BCR (a-BCR) (Santa Cruz Biotechnology), anti-phosphorylated BCR-Y177 (a-p-BCR-Y177) (Cell Signaling, Boston, USA), anti-STAT5, anti phosphorylated STAT5 (Cell Signaling, Boston, MA, USA), anti-Erk1/2 and anti phosphorylated Erk1/2 (Cell Signaling, Boston, MA, USA).

Techniques: Western Blot, Expressing, Control

Journal: Genes & Cancer

Article Title: BCR: a new target in resistance mediated by BCR/ABL-315I?

doi:

Figure Lengend Snippet: 32D cells were transduced with the indicated transgenes and retransduced lentivirally with different shRNAs against endogenous BCR. A. Western blotting on lysates of 32D cells expressing the indicated transgenes using antibodies directed against BCR. Molecular mass references (KDa) are given and Tubulin was used as a loading control. B. For determining cells proliferation, XTT assay was performed. C. The apoptosis rate was determined by staining with 7-AAD. The data represent the mean and SD of three independent experiments. D. Western blotting on lysates of 32D cells expressing the indicated transgenes using antibodies directed against Erk1/2 and phosphorylated Erk1/2. Molecular mass references (KDa) are given.

Article Snippet: The following antibodies were used: anti-ABL (a-ABL) Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-phosphorylated ABL specific for the phosphorylated tyrosine-residues 245 (a-p-ABL-Y245) (Upstate-Biotechnology, Lake Placid, NY, USA), anti-BCR (a-BCR) (Santa Cruz Biotechnology), anti-phosphorylated BCR-Y177 (a-p-BCR-Y177) (Cell Signaling, Boston, USA), anti-STAT5, anti phosphorylated STAT5 (Cell Signaling, Boston, MA, USA), anti-Erk1/2 and anti phosphorylated Erk1/2 (Cell Signaling, Boston, MA, USA).

Techniques: Transduction, Western Blot, Expressing, Control, XTT Assay, Staining